VDJ Recombination

Finally, the mRNA is translated into a light-chain polypeptide by ribosomes.

The variable region of the immunoglobulin light chain is formed by V and J gene segments through the VDJ recombination process.

Introns are removed via RNA splicing, and the variable region exon, formed by the V and J gene segments, is joined to the C region exon. Polyadenylation occurs, producing mRNA.

VDJ recombination is a process in B-cells that assembles immunoglobulin gene segments to form functional variable region exons, contributing to antibody diversity.

Light chains are composed of V, J, and C gene segments. The V and J segments combine to encode the variable region of the light chain. An example is the Kappa light chain locus, consisting of V gene segments with leader sequences, J gene segments, and a C gene.

The first step involves gene rearrangement, also known as somatic DNA recombination, which occurs only in somatic cells. A randomly selected V gene segment joins a randomly selected J gene segment.

The rearranged DNA is then transcribed into pre-RNA, or primary transcript RNA.