Aseptic Technique video protocol

Before starting work, ensure clean hands and proper PPE (gloves, lab coat, safety glasses). Thoroughly disinfect the cell culture hood with disinfectant and then 70% ethanol, wiping clean with tissue after each spray.

Retrieve cell culture media and supplements from the fridge and warm them in a 37-degree incubator. Aseptically prepare media by spraying each item with ethanol before use. Work quickly and efficiently, arranging bottles for easy access.

Add supplements using a new sterile tip or pipette. Only open bottles immediately before use, and if a lid is placed down, ensure the opening faces downwards. Never sweep hands or sleeves over open bottles. Replace lids promptly after use to minimize contamination risk.

Label media bottles with your initials, the date, and details of their contents.