Aseptic Technique video protocol

Preparation and Hood Cleaning00:00:17

Before starting work, ensure clean hands and proper PPE (gloves, lab coat, safety glasses). Thoroughly disinfect the cell culture hood with disinfectant and then 70% ethanol, wiping clean with tissue after each spray.

Media Preparation and Handling00:00:55

Retrieve cell culture media and supplements from the fridge and warm them in a 37-degree incubator. Aseptically prepare media by spraying each item with ethanol before use. Work quickly and efficiently, arranging bottles for easy access.

Avoiding Contamination00:01:34

Add supplements using a new sterile tip or pipette. Only open bottles immediately before use, and if a lid is placed down, ensure the opening faces downwards. Never sweep hands or sleeves over open bottles. Replace lids promptly after use to minimize contamination risk.

Labeling Media00:02:14

Label media bottles with your initials, the date, and details of their contents.