Summary
Highlights
Preparation and Hood Cleaning00:00:17
Before starting work, ensure clean hands and proper PPE (gloves, lab coat, safety glasses). Thoroughly disinfect the cell culture hood with disinfectant and then 70% ethanol, wiping clean with tissue after each spray.
Media Preparation and Handling00:00:55
Retrieve cell culture media and supplements from the fridge and warm them in a 37-degree incubator. Aseptically prepare media by spraying each item with ethanol before use. Work quickly and efficiently, arranging bottles for easy access.
Avoiding Contamination00:01:34
Add supplements using a new sterile tip or pipette. Only open bottles immediately before use, and if a lid is placed down, ensure the opening faces downwards. Never sweep hands or sleeves over open bottles. Replace lids promptly after use to minimize contamination risk.
Labeling Media00:02:14
Label media bottles with your initials, the date, and details of their contents.