BSN LAS 2 Lipids part 2 POSTLAB

Salkowski and Libermann-Burchard Tests00:00:04

These tests detect cholesterol. The Salkowski test uses sulfuric acid to form a colored ring (reddish-brown or purple), while the Libermann-Burchard test uses acetic anhydride, resulting in a color shift that stabilizes into an emerald or blue-green color.

Molisch and Kraut's Tests00:04:05

The Molisch test identifies sugars/monosaccharides within a lipid solution using sulfuric acid. The Kraut's test detects choline-containing lipids like lecithin or sphingomyelin, producing an orange-red solution that turns into a brick-red precipitate upon boiling.

Ninhydrin and Acrolein Tests00:05:34

The Ninhydrin test identifies amino lipids (like cephalin) by producing a deep blue or purple color. The Acrolein test detects glycerol-based lipids; heating the sample transforms glycerol into acrolein, which is identified by a distinct, pungent, burnt-oil odor.